| Reverse contig does not mean for us
that a contig's sequence is in reverse (because
soybean contigs have not been sequenced). Rather,
reverse contig refers to a possible
alternate location that a contig might have if it
were reversed. See the image below as an
illustration.

In the above image, a locus anchors a clone which
indirectly anchors a contig. This clone is in the
first few beginning bands of the contig, according
to the FPC program. We call this default FPC
placement the forward contig. The example contig in
the above image happends to extends out to the right
of the locus in this default FPC placement.
However, the default FPC placement could be
incorrect. The example clone could possibly be in
the last few bands of the contig instead of being in
the first few bands, as illustrated in the image
above. We call this alternate placement the reverse
contig. In the above example, the reverse contig
extends out to the left of the locus.
If the clone anchor is in the middle of the
contig, then the forward and reverse locations would
be the same.
If a contig has more than one clone anchor, then
it is possible that only the forward direction, or
only the reverse direction, is appropriate. In our
database, if the reverse contig is the only
appropriate direction, we call the contig
flipped. |